DIR6 Antibody

Code CSB-PA821069XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DIR6 Polyclonal antibody
Uniprot No.
Target Names
DIR6
Alternative Names
DIR6 antibody; At4g23690 antibody; F9D16.160Dirigent protein 6 antibody; AtDIR6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DIR6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism. Enantiocomplementary dirigent protein that mediates the laccase-catalyzed enantioselective oxidative phenol coupling of (E)-coniferyl alcohol to (-)-pinoresinol.
Gene References into Functions
  1. the crystal structure of the (-)-pinoresinol forming DIRIGENT PROTEIN6 (AtDIR6) from Arabidopsis (Arabidopsis thaliana), is reported. PMID: 27756822
  2. Authors demonstrate that the glycan structures of AtDIR6 are essential for structure, solubility, and function of the protein as deglycosylation induced conformational changes leading to the complete loss in dirigent activity and subsequent protein aggregation.[DIR6] PMID: 23188459
Subcellular Location
Secreted, extracellular space, apoplast.
Protein Families
Plant dirigent protein family
Tissue Specificity
Expressed in roots, cotyledon veins, leaf trichomes, flowers, siliques, and meristems. Present in interfascicular/vascular cambia and developing xylem.
Database Links

KEGG: ath:AT4G23690

STRING: 3702.AT4G23690.1

UniGene: At.20823

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