Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans dlk-1 Polyclonal antibody

Uniprot No.

O01700

Target Names

dlk-1

Alternative Names

dlk-1 antibody; F33E2.2Mitogen-activated protein kinase kinase kinase dlk-1 antibody; EC 2.7.11.25 antibody; DAP kinase-like kinase antibody; Death-associated protein kinase-like kinase antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans dlk-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of a MAP kinase pathway that functions presynaptically to regulate synaptic architecture and presynaptic differentiation. Phosphorylates and activates mkk-4. Has a role in axonal regrowth following injury and synaptogenesis. Also promotes tubulin post-translational modifications that protect microtubules. Plays a role in cilium length regulation, possibly by reducing rab-5 mediated endocytosis, and may also have a role in intraflagellar transport in cilia. Plays a role in the formation of muscle connections, also called muscle arm extensions, between the body wall and the motor axons in the dorsal and ventral cord.; Has a role in synapse and axon development, and in axonal regrowth following injury.; By forming heterooligomers with isoform a, acts as an inhibitor of isoform a activation. Its inhibitory function is independent of its catalytic activity.

Gene References into Functions

Mitochondrial localization to the axon after injury is regulated in part by dual-leucine zipper kinase 1 (DLK-1), a conserved regulator of axon regeneration. PMID: 28009276
DLK-1, SEK-3 and PMK-3 have roles in C. elegans adaptation to disruption of the mitochondrial electron transport chain, and in extended lifespan PMID: 27420916
These findings explain how DLK specifically mediates nerve injury responses and reveal a novel cellular mechanism that ensures the specificity of neuronal kinase signaling. PMID: 26719418
Independent of myosin light chain activation, RHGF-1 acts via Rho-dependent kinase LET-502/ROCK and activates a conserved, retrograde DLK-1 MAPK pathway, triggering synaptic branch retraction and overgrowth of the PLM neurite in a dose-dependent manner. PMID: 25359212
Genetic, transgenic, and biochemical studies indicated that PPM-2 functions coordinately with the ubiquitin ligase activity of RPM-1 and the F-box protein FSN-1 to negatively regulate DLK-1 PMID: 24810406
DLK-1 is activated by calcium-mediated dissociation from an inhibitory isoform. PMID: 23141066
These findings reveal the relationship between Ca(2+)/cAMP signaling and the DLK-1 MAPK (mitogen-activated protein kinase) cascade in regeneration. PMID: 20203177
findings show that the DLK-1 mitogen-activated protein (MAP) kinase pathway is essential for regeneration in Caenorhabditis elegans motor neurons PMID: 19164707
Understanding how the DLK-1 pathway acts in development, plasticity, and regeneration may shed light on the evolution of mechanisms regulating axon regeneration. PMID: 19417215
Study shows that in C. elegans DLK-1 regulates not only proper synapse formation and axon morphology but also axon regeneration by influencing mRNA stability. PMID: 19737525

Hide All

Subcellular Location

Cell junction, synapse. Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell projection, cilium.; [Isoform a]: Cell junction, synapse. Cell projection, axon.; [Isoform c]: Cell junction, synapse. Cell projection, axon.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily

Tissue Specificity

Expressed in nerve ring, nerve cord, neurons, and pharynx.

Database Links

KEGG: cel:CELE_F33E2.2

STRING: 6239.F33E2.2a

UniGene: Cel.24063

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA516860XA01CXY
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

dlk-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background