dnaA Antibody

1. analysis of individual DnaA protomers in a complex suggests a mechanism that might support timely assembly of ATP-DnaA on oriC PMID: 29040689
2. a mechanistic model of datA-IHF.DnaA complex formation and DNA supercoiling-dependent regulation for DDAH. PMID: 27941026
3. In Escherichia coli, DnaA also shows low residence times in the range of 200 ms and oscillates between spatially opposite chromosome regions in a time frame of one to two seconds, independently of ongoing transcription. PMID: 28166228
4. Trans-translation ensures timely initiation of DNA replication and DnaA synthesis in Escherichia coli. PMID: 27706629
5. Cellular acidic phospholipids may positively and negatively influence the initiation activity of DnaA protein to help assure chromosomal replication occurs once, but only once, per cell-cycle. Review. PMID: 23595001
6. In cells where datA was deleted or an excess of DnaA was provided, cell division occurred in the absence of replication and anucleate cells were formed. PMID: 24574433
7. The study of mutant DnaAs that are refractory to negative control provide new insight into the mechanisms how DnaA helps regulate replication initiation in Escherichia coli. PMID: 23990329
8. Data indicate a positive autoregulation of dnaA expression in E. coli. PMID: 23092251
9. second DnaA-ATP hydrolysis system that occurs at the chromosomal site datA. This locus has been annotated as a reservoir for DnaA that binds many DnaA molecules in a manner dependent upon the nucleoid-associated factor IHF PMID: 23277577
10. DnaA binds to two oppositely oriented helically phased arrays of low-affinity sites in oriC. PMID: 21895796
11. Two suppressor mutations in the dnaA gene led to a reduced ability to initiate replication from oriC. PMID: 21299647
12. A role for nonessential domain II of initiator protein, DnaA, in replication control. PMID: 19546317
13. DnaA oligomerization at the E. coli replication origin requires the box VII motif of DnaA protein PMID: 15371441
14. Mutant dnaA is degraded by protein encoded by hspQ.- a novel heat shock protein. PMID: 15569148
15. residues that might be involved specifically in the cooperative interaction with ATP-DnaA boxes PMID: 15588821
16. DnaA bound to oriC forms a specific oligomeric structure, which is required to load DnaB helicase PMID: 15878847
17. the DnaA Arg-285 residue plays a role in the ATP-dependent conformational activation of an initial complex by recognizing ATP bound to DnaA and by modulating the structure of the DnaA multimer to allow interaction with ATP-DnaA-specific binding sites PMID: 15901724
18. coordinated sequestration of oriC and dnaA is required for maintaining controlled once-per-cell-cycle initiation PMID: 16077105
19. results suggest that mutant DnaAs with A213D & T435K substitutions suppress the temperature-sensitive phenotype of a dnaX mutant by reducing the frequency of initiation and, therefore, chromosome content per cell PMID: 16225979
20. two functional states of DnaA exist at high and low membrane occupancy, ascribed to local macromolecular crowding on the membrane surface PMID: 16517983
21. A new set of rules for identifying efficiently titrating DnaA box regions was formulated and used to analyse sequences for which good titration data are available. PMID: 17316685
22. These findings suggest a model for DnaA homomultimer formation and DnaB helicase loading on oriC. PMID: 17420252
23. Results demonstrate that mutant DnaA may be activated at significantly lower concentrations of acidic phospholipids than the wild-type protein. PMID: 17719583
24. Results show that both DnaA-ATP and DnaA-ADP interacts with Escherichia coli oriC during pre-RC assembly. PMID: 17850252
25. observations support a model whereby DnaA interacts with the HU alpha dimer or the alphabeta heterodimer, depending on their cellular abundance, to recruit the respective form of HU to oriC PMID: 18179598
26. ATP-DnaA-oriC unwinding element complexes specifically bound the upper strand of single-stranded DUE PMID: 18216012
27. Data report that Dps interacts with DnaA and requires the N-terminal region of DnaA to interact. In vitro, Dps inhibits DnaA function in initiation by interfering with strand opening of the replication origin. PMID: 18284581
28. A GATC cluster inserted at the datA site, which binds large amounts of DnaA in vivo, did not become remethylated at all, unless the availability of the DnaA protein was severely reduced. PMID: 18835566
29. The 79-135 and 21-27 residues of Domain II of E. coli DnaA are required cell viability. PMID: 18957591
30. Replication initiation in DnaA-box R4 mutants is insensitive to increased levels of cellular DnaA-ATP. PMID: 19007419
31. The DnaA protein interacted with RNA polymerase and that it afforded a partial protection from the negative effect of rifampicin. PMID: 19170875
32. Surplus DnaAcos hyperinitiates DNA Replication and causes lethality at 30degC, 37degC and 42degC, whereas excess DnaA is toxic only at 37degC and 42degC but not at 30degC in a repair-defective strain. PMID: 19432804
33. Study show that replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle. PMID: 19502409
34. assignments of 1H, 13C, and 15N resonances of N-terminal domain of Dna A (1-108) which contains the activities of self-oligomerization and DnaB helicase loading PMID: 19636826

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KEGG: ecj:JW3679

STRING: 316407.85676342