dnaJ Antibody

Code CSB-PA484226XA01EOR
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Product Details

Full Product Name
Rabbit anti-Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) dnaJ Polyclonal antibody
Uniprot No.
Target Names
dnaJ
Alternative Names
dnaJ antibody; EFER_0011Chaperone protein DnaJ antibody
Raised in
Rabbit
Species Reactivity
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Immunogen
Recombinant Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) dnaJ protein
Immunogen Species
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.
Subcellular Location
Cytoplasm.
Protein Families
DnaJ family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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