ATJ15 Antibody

Code CSB-PA449873XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ATJ15 Polyclonal antibody
Uniprot No.
Target Names
ATJ15
Alternative Names
ATJ15 antibody; ARG1 antibody; B15 antibody; At1g68370 antibody; T2E12.8 antibody; Chaperone protein dnaJ 15 antibody; AtDjB15 antibody; AtJ15 antibody; Protein ALTERED RESPONSE TO GRAVITY antibody; AtARG1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ATJ15 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Plays a continuous role in plant development probably in the structural organization of compartments. Seems to be involved in early gravitropic signal transduction within the gravity-perceiving cells (statocytes), where it influences pH changes and auxin distribution. Probably affects the localization and/or activity of auxin efflux carrier components (PIN proteins) or other proteins involved in lateral auxin transport.
Gene References into Functions
  1. Most of the genes altered in expression in spaceflight in WT cells were found to be Arg1-dependent, suggesting a major role for that gene in the physiological adaptation of undifferentiated cells to spaceflight. PMID: 29088549
  2. Under NH4(+) stress, Arabidopsis mutant gsa-1 (gravitropism sensitive to ammonium-1) displayed increased loss of root gravitropism. And, disruption of GSA-1/ARG1 can reduce basipetal auxin transport and the expression of AUX1 protein in root apices. [ARG1] [ALTERED RESPONSE TO GRAVITY1] PMID: 23782229
Subcellular Location
Cytoplasm, cytoskeleton. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Note=Found in endoplasmic reticulum, Golgi, vesicles near the plasma membrane and around cell plate, and bound to the plasma membrane. Probably interacts with integral membrane proteins. Also interacts with cytoskeleton.
Protein Families
DnaJ family, B/II subfamily
Tissue Specificity
Expressed at high levels in root cap, root tip meristematic region and elongation zones, and at lower levels in mature part of roots (at protein level). Constitutively expressed in seedlings, etiolated or not, roots, rosette leaves, cauline leaves, stems,
Database Links

KEGG: ath:AT1G68370

STRING: 3702.AT1G68370.1

UniGene: At.11013

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