CDC9 Antibody

Code CSB-PA012930XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CDC9 Polyclonal antibody
Uniprot No.
Target Names
CDC9
Alternative Names
CDC9 antibody; YDL164CDNA ligase 1 antibody; EC 6.5.1.1 antibody; DNA ligase I antibody; Polydeoxyribonucleotide synthase [ATP] 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CDC9 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. The mitochondrial form is required for mitochondrial DNA maintenance but is non-essential while the nuclear form is essential for cell viability.
Gene References into Functions
  1. Rad27 lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. PMID: 19596905
  2. Higher than normal levels of Cdc9p expression can yield genetic instability by disrupting the normal interplay of PCNA with other proteins such as Fen1. PMID: 15965249
  3. Combining rad27-p with cdc9-p increases the incidence of repeat tract expansion frequency above the sum of the numbers recorded in the individual mutants PMID: 16079237
  4. Recruitment of Saccharomyces cerevisiae Dnl4-Lif1 complex to a double-strand break requires interactions with Yku80 and the Xrs2 FHA domain. PMID: 18832348
Subcellular Location
[Isoform Mitochondrial]: Mitochondrion.; [Isoform Nuclear]: Nucleus.
Protein Families
ATP-dependent DNA ligase family
Database Links

KEGG: sce:YDL164C

STRING: 4932.YDL164C

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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