DMT1 Antibody

Code CSB-PA335990XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DMT1 Polyclonal antibody
Uniprot No.
Target Names
DMT1
Alternative Names
DMT1 antibody; ATHIM antibody; DDM2 antibody; DMT01 antibody; MET1 antibody; MET2 antibody; At5g49160 antibody; K21P3.3DNA antibody; cytosine-5)-methyltransferase 1 antibody; EC 2.1.1.37 antibody; DNA methyltransferase 01 antibody; DNA methyltransferase 2 antibody; DNA methyltransferase AthI antibody; DNA Metase AthI antibody; M.AthI antibody; DNA methyltransferase DDM2 antibody; Protein DECREASED DNA METHYLATION 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DMT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. Required for proper patterns of CG DNA methylation in dividing cells. Required for MEA promoter methylation in seeds.
Gene References into Functions
  1. The findings reveal that MET1-mediated shoot regeneration is regulated by the cytokinin-induced cell cycle, and provide new insights into the regulation of DNA methylation in shoot regeneration. PMID: 28960381
  2. Long-term stability and epigenetic features differ for individual loci. Our data show that over-expression of MET1, and potentially of other genes encoding epigenetic factors, offers an alternative strategy to identify epigenetic target genes and to create novel epi-alleles PMID: 29466369
  3. MET1 is a thylakoid-associated TPR protein involved in photosystem II supercomplex formation and repair in Arabidopsis PMID: 25587003
  4. Met1 gene expression throughout normal development, particularly in the flower PMID: 25842863
  5. MET1 is a contributor to epigenetic diversity in Arabidopsis. PMID: 25137154
  6. VIM proteins regulate genome-wide epigenetic gene silencing through coordinated modulation of DNA methylation and histone modification status in collaboration with MET1 PMID: 25009302
  7. Genetic studies indicate that the Polycomb Repressive Complex 2 (PRC2) but not the DNA METHYLTRANSFERASE1 (MET1) is involved in regulating imprinted expression in the embryo. [MET1] PMID: 24339783
  8. MET1 restores body methylation, which is region-specific but random with respect to the affected CG sites, and is moderately although not decisively influenced by transcription. PMID: 22587613
  9. There is a mechanistic link between two major epigenetic pathways involved in histone and DNA methylation in plants by physical interaction of MET1 with the FIS-PRC2 core component MEA. PMID: 23146178
  10. Our results bear interesting similarities with cancer cells, which show global losses of DNA methylation but ectopic hypermethylation of genes previously marked by H3K27m3. PMID: 23209430
  11. An intergenic nucleosome-free crossover hotspot 3a undergoes increased recombination activity in met1. PMID: 22876192
  12. HDA6 and MET1 interact directly and act together to silence transposable elements by modulating DNA methylation, histone acetylation, and histone methylation status. PMID: 21994348
  13. functional loss of key epigenetic genes-including METHYLTRANSFERASE1 (MET1) encoding for DNA methyltransferase, in Arabidopsis PMID: 21876682
  14. The methylation patterns of the MET1 gene in organs of Arabidopsis thaliana were studied. PMID: 21542302
  15. Maternal and paternal genomes play distinct roles in the regulation of seed size in Arabidopsis, as determined by mutation status of DDM1 and MET1. PMID: 17012404
  16. Continuous MET1 activity in dividing cells is essential for proper patterns of CG DNA methylation & development. PMID: 18820427

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Subcellular Location
Nucleus.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family
Tissue Specificity
Expressed in flowers, embryos and endosperm.
Database Links

KEGG: ath:AT5G49160

STRING: 3702.AT5G49160.1

UniGene: At.20294

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