dnrK Antibody

Code CSB-PA981904XA01SPD
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Product Details

Full Product Name
Rabbit anti-Streptomyces peucetius dnrK Polyclonal antibody
Uniprot No.
Target Names
dnrK
Alternative Names
dnrKCarminomycin 4-O-methyltransferase DnrK antibody; COMT antibody; EC 2.1.1.292 antibody; Anthracycline 4-O-methyltransferase antibody
Raised in
Rabbit
Species Reactivity
Streptomyces peucetius
Immunogen
Recombinant Streptomyces peucetius dnrK protein
Immunogen Species
Streptomyces peucetius
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family
Database Links

KEGG: ag:AAA26712

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