DOF5.1 Antibody

Code CSB-PA873411XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DOF5.1 Polyclonal antibody
Uniprot No.
Target Names
DOF5.1
Alternative Names
DOF5.1 antibody; PEAR2 antibody; At5g02460 antibody; T1E22.3 antibody; T22P11_50Dof zinc finger protein DOF5.1 antibody; AtDOF5.1 antibody; Protein PHLOEM EARLY DOF 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DOF5.1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. Binds to 5'-TAAAGT-3' motif in REV promoter to triggers its transcription, thus regulating adaxial-abaxial polarity and influencing leaf axial patterning in an auxin transport- and response-dependent manner (e.g. IAA6 and IAA19 genes expression). Probably involved in early processes for vascular development. The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) activate gene expression that promotes radial growth of protophloem sieve elements.
Gene References into Functions
  1. Dof5.1 protein regulates leaf adaxial-abaxial polarity by controlling REV expression. PMID: 20807212
Subcellular Location
Nucleus.
Tissue Specificity
Expressed ubiquitously, especially in the vascular tissues, except in seeds, petals and anthers. Specific to the vascular tissues in young leaves, cotyledons and flower buds. The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) form
Database Links

KEGG: ath:AT5G02460

STRING: 3702.AT5G02460.1

UniGene: At.33412

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