DOG1 Antibody

Code CSB-PA843913XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DOG1 Polyclonal antibody
Uniprot No.
Target Names
DOG1
Alternative Names
DOG1 antibody; GSQ5 antibody; At5g45830 antibody; K15I22.3Protein DELAY OF GERMINATION 1 antibody; GLUCOSE SENSING QTL 5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DOG1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the induction of seed dormancy. The level of DOG1 protein in freshly harvested seeds determines the level of seed dormancy. Determines the temperature window for germination by regulating the expression of micropylar endosperm-weakening genes through temperature control of the gibberellins metabolism. Regulates seed dormancy and flowering time through an influence on levels of microRNAs miR156 and miR172. Regulator of seed maturation interfering with abscisic acid signaling components and activating ABI5. In cv. Cvi-1, enhances glucose induction of ABI4.
Gene References into Functions
  1. Data show that that ABA HYPERSENSITIVE GERMINATION1 (AHG1) interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. PMID: 29875377
  2. reveals reciprocal regulation of the DOG1-asDOG1 pair PMID: 28782720
  3. Here, we show that asDOG1 shows high levels in mature plants where it suppresses DOG1 expression under standard growth conditions. Suppression is released by shutting down antisense transcription, which is induced by the plant hormone abscisic acid (ABA) and drought. PMID: 29030481
  4. Antisense (as)DOG1 strongly suppresses DOG1 expression during seed maturation in cis, but is unable to do so in trans Therefore, the negative regulation of seed dormancy by asDOG1 in cis results in allele-specific suppression of DOG1 expression and promotes germination. PMID: 27856735
  5. The results suggest a functional role for some components of the circadian clock related with the action of DOG1 for the integration of alternating temperatures and light signals in the relief of seed dormancy. PMID: 28941290
  6. DOG1 can interact with the type 2C protein phosphatases AHG1 and AHG3 and this represents the convergence point of the DOG1-regulated dormancy pathway and signalling by the plant hormone abscisic acid. PMID: 28706187
  7. using mutants in known dormancy-related genes [DELAY OF GERMINATION 1 (DOG1), MOTHER OF FLOWERING TIME (MFT), CBL-INTERACTING PROTEIN KINASE 23 (CIPK23) and PHYTOCHROME A (PHYA)] components of the circadian clock are shown to be involved in coordination of the annual seed dormancy cycle. PMID: 28240777
  8. regulatory mutations are likely to provide a quantitative molecular mechanism for the adaptation of life cycle to altitude and climate PMID: 26991665
  9. higher miR156 levels resulting from overexpression of the MIR156 gene enhanced seed dormancy and delayed flowering. These phenotypic effects, as well as conversion of MIR156 transcripts to miR156, were compromised in DOG1 loss-of-function mutant plants, especially in seeds. Overexpression of MIR172 reduced seed dormancy and promoted early flowering PMID: 27035986
  10. the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis. PMID: 26620523
  11. DOG1 protein can bind to itself and that this binding is required for DOG1 function but not for protein accumulation. Natural variation for DOG1 binding efficiency was observed among Arabidopsis accessions and contributes to variation in seed dormancy PMID: 26684465
  12. Changes in the histone marks H3K4me3 and H3K27me3 are seen on DOG1 (DELAY OF GERMINATION1) during dormancy cycling. PMID: 25439058
  13. The DOG1 gene determines the optimal temperature for seed germination. Reciprocal gene-swapping experiments between Brassicaceae species show that the DOG1-mediated dormancy mechanism is conserved. PMID: 25114251
  14. Soil temperature drives seed-specific temporal sensing via the accumulation of DELAY OF GERMINATION 1 (DOG1) protein to drive changes in germination potential. PMID: 23590427
  15. The DOG1 protein accumulates during seed maturation and remains stable throughout seed storage and imbibition. DOG1 functions independent from Abscisic acid (ABA). The presence of both DOG1 and ABA is required for seed dormancy. PMID: 22829147
  16. Patterns of genetic differentiation among populations suggest that the gene delay of germination 1 (DOG1) contributes to local adaptation. A correlation with variation in summer precipitation supports that seed dormancy is adaptive. PMID: 22759302
  17. Data show that C-REPEAT BINDING FACTORS (CBFs) are necessary for regulation of dormancy and of GA2OX6 and DOG1 expression caused by low temperatures. PMID: 21803937
  18. The analysis revealed quantitative variation for seed dormancy that is associated with allelic variation at the seed dormancy QTL DOG1 (for Delay Of Germination 1) in one population. PMID: 21738591
  19. DOG1 expression is associated with seed-maturation temperature effects on germination PMID: 21740475
  20. DOG1 is associated with sugar sensitivity and the induction of ABI4. PMID: 18410483

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Subcellular Location
[Isoform 1]: Nucleus.; [Isoform 2]: Nucleus.
Tissue Specificity
Detected only in seeds. Expressed mainly in vascular tissues of the cotyledon, hypocotyl and radicle of the embryo.
Database Links

KEGG: ath:AT5G45830

STRING: 3702.AT5G45830.1

UniGene: At.29992

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