nero Antibody

Code CSB-PA892698XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) nero Polyclonal antibody
Uniprot No.
Target Names
nero
Alternative Names
nero antibody; l(3)s1921 antibody; CG2245Deoxyhypusine hydroxylase antibody; DOHH antibody; EC 1.14.99.29 antibody; Deoxyhypusine dioxygenase antibody; Deoxyhypusine monooxygenase antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) nero protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. Essential for organismal viability and plays a role in a wide number of important processes such as cell growth and proliferation, and regulates induction of autophagy and protein synthesis. Has a role in eIF-5A-mediated translational control.
Gene References into Functions
  1. Results describe the identification and characterization of nero (CG2245), the Drosophila melanogaster deoxyhypusine hydroxylase (DOHH) homologue, and indicate that nero and eIF5A are required for cell growth and affect autophagy and protein synthesis. PMID: 19546244
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In larval imaginal disk and Garland cells.
Protein Families
Deoxyhypusine hydroxylase family
Database Links

KEGG: dme:Dmel_CG2245

STRING: 7227.FBpp0085222

UniGene: Dm.29814

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