dome Antibody

Code CSB-PA894285XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) dome Polyclonal antibody
Uniprot No.
Target Names
dome
Alternative Names
dome antibody; CG14226Cytokine receptor antibody; Protein domeless antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) dome protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Critical for epithelial morphogenesis during oogenesis; border cell migration. Required in the germarium for the polarization of follicle cells during encapsulation of germline cells. Required for embryonic segmentation and trachea specification. Essential receptor molecule for upd and JAK/STAT signaling during oogenesis.
Gene References into Functions
  1. ectopic expression of Wdp inhibits JAK/STAT signaling activity. Importantly, we show that Wdp interacts with the receptor Domeless (Dome), and promotes its internalization for subsequent lysosomal degradation PMID: 25923769
  2. Drosophila epithelia lacking Hrs and Stam, accumulate Dome in endosomes. PMID: 24718108
  3. Data indicate that Domeless is an essential receptor molecule for Upd and JAK/STAT signaling during oogenesis. PMID: 12403714
  4. ligand-independent dimerisation of the JAK/STAT receptor DOME confers cells with competence to activate the pathway prior to ligand reception PMID: 12783781
  5. Awd mutant clones in the main-body follicle cells exhibited significant overaccumulation of Dome, confirming that Awd is a negative regulator of Dome expression. PMID: 18212059

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Subcellular Location
Apicolateral cell membrane; Single-pass type I membrane protein. Note=Apicolateral membranes in follical cells.
Protein Families
Type I cytokine receptor family
Tissue Specificity
In stage 11 embryos, tracheal pits show highest expression, at stage 14 high expression is detected in the posterior spiracles, gut and head.
Database Links

KEGG: dme:Dmel_CG14226

STRING: 7227.FBpp0074525

UniGene: Dm.2836

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