Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) dosP Polyclonal antibody

Uniprot No.

P76129

Target Names

dosP

Alternative Names

dosP antibody; dos antibody; pdeO antibody; yddU antibody; b1489 antibody; JW1484Oxygen sensor protein DosP antibody; EC 3.1.4.52 antibody; Direct oxygen-sensing phosphodiesterase antibody; Direct oxygen sensor protein antibody; Ec DOS antibody; Heme-regulated cyclic di-GMP phosphodiesterase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) dosP protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Heme-based oxygen sensor protein displaying phosphodiesterase (PDE) activity toward c-di-GMP in response to oxygen availability. Involved in the modulation of intracellular c-di-GMP levels, in association with DosC which catalyzes the biosynthesis of c-di-GMP (diguanylate cyclase activity). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. Has very poor PDE activity on cAMP but is not active with cGMP, bis(p-nitrophenyl) phosphate or p-nitrophenyl phosphate. Via its PDE activity on c-di-GMP, DosP regulates biofilm formation through the repression of transcription of the csgBAC operon, which encodes curli structural subunits.

Gene References into Functions

c-di-GMP positively modulates PgaD protein stability in a PgaC-dependent manner. PMID: 23202856
A phosphodiesterase (PDE) from Escherichia coli (Ec DOS) is a novel haem-based oxygen sensor enzyme. PMID: 20861024
These results suggest that YddV and Dos, through their opposite activities, fine-tune curli production in response to oxygen availability. PMID: 20553324
expression of DOS(Ec) is regulated according to environmental O2 availability at the transcriptional level and that the concentration of cAMP in cells is regulated by DOS(Ec) expression PMID: 16166529
It appears that Leu99 and Leu115 are critical for determining the characteristics of heme iron. Finally, we discuss the roles of these amino-acid residues in the heme electronic states. PMID: 16519686
importance of the hydrogen-bonding network from heme 6-propionate to Tyr126 through Asn84 in signal transmission PMID: 17335280
the heme axial ligand, Met95, has a critical role in locking catalysis of the phosphodiesterase from Escherichia coli (Ec DOS) toward Cyclic diGMP PMID: 17535805
Data suggest that Arg97 plays critical roles in recognition of the O2 molecule and redox switching by stabilizing the Fe(II)-O2 complex. PMID: 18672892
Examination of the catalytic activity of ferric complex-bound full-length Ec DOS in absence and presence of external ligands cyanide and imidazole shows that the heme at the sensor domain inhibits catalysis. PMID: 19053256
Phe113, residing near the O(2) molecule, has a critical role in optimizing the Fe(II)-O(2) complex for effective regulation of catalysis by DOS. PMID: 19482359

Hide All

Database Links

KEGG: ecj:JW1484

STRING: 316385.ECDH10B_1620

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dosP Antibody

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Target Background