DPB11 Antibody

Code CSB-PA344426XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DPB11 Polyclonal antibody
Uniprot No.
Target Names
DPB11
Alternative Names
DPB11 antibody; YJL090C antibody; J0918DNA replication regulator DPB11 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DPB11 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Required for the association of PSF1 with origins. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Multicopy suppressor of DPB2 mutation. Overexpression restores the growth defect conferred by POL2 mutation.
Gene References into Functions
  1. The ddc2-S4 mutation decreases phosphorylation of histone H2A more significantly than the absence of Ddc1 and Dpb11 function does. PMID: 24586187
  2. Depletion of TopBP1/Dpb11 led to an accumulation of chromatin bridges. PMID: 24379413
  3. the inter-BRCT region of Dpb11 is a functionally conserved GINS interaction domain that is important for the initiation of DNA replication PMID: 23629628
  4. Dpb11 plays distinct roles in replication, checkpoint response and recombination processes, thereby contributing to chromosomal stability PMID: 21130053
  5. CDK promotes the formation of a newly identified fragile complex, the preloading complex (pre-LC) containing DNA polymerase epsilon (Pol epsilon), GINS, Sld2, and Dpb11 PMID: 20231317
  6. Phosphorylation of canonical motifs in Sld2 does not play a direct role in complex formation with Dpb11, but rather regulates phosphorylation of another residue, Thr84. PMID: 16619031
  7. Dpb11 functions with Ddc1 in the recombination repair process. PMID: 16840526
  8. Dpb11 is held in proximity to damaged DNA through an interaction with the phosphorylated 9-1-1 complex, leading to Mec1-dependent phosphorylation of Rad9. PMID: 18541674
  9. MSA1 is a high copy suppressor of a drc1-1 mutant. PMID: 18948746

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YJL090C

STRING: 4932.YJL090C

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