POL4 Antibody

Code CSB-PA340715XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) POL4 Polyclonal antibody
Uniprot No.
Target Names
POL4
Alternative Names
POL4 antibody; POLX antibody; YCR014C antibody; YCR14CDNA polymerase IV antibody; POL IV antibody; EC 2.7.7.7 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) POL4 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Repair polymerase. Involved in gap-filling in DNA nonhomologous end joining (NHEJ) required for double-strand break repair. Seems to conduct DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Preferentially acts upon short gaps formed by the alignment of linear duplexes with complementary single-strand ends. Required for filling gaps that need removal of a 5'- or 3'-terminal mismatch, however lacks nuclease activities.
Gene References into Functions
  1. Our data suggest that the role of Tel1 in preventing break-induced chromosomal translocations can, to some extent, be due to its stimulating effect on gap-filling activity of Pol4 to repair DSBs in cis PMID: 23874240
  2. both yeast and human pol eta synthesize past the 3'-(m)C CPD in a >99% error-free manner, consistent with the highly water-exposed nature of the active site. PMID: 22262850
  3. Results describe a series of pair-wise interactions among FEN-1, Pol4 and Dnl4, the factors that complete the repair of DNA double-strand breaks by non-homologous end-joining. PMID: 15342630
  4. possible role for pol IV in base excision repair PMID: 15778218
  5. Pol4 is exclusively associated with the NHEJ pathway of double-stranded break repair PMID: 15964833
  6. Pol4 is able to extend a 3'-end whose last bases are mismatched to the template strand PMID: 16452137
  7. model predicts that a unique function of Pol4 is to actively stabilize template strands that lack stacking continuity PMID: 17881298

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Subcellular Location
Nucleus.
Protein Families
DNA polymerase type-X family
Database Links

KEGG: sce:YCR014C

STRING: 4932.YCR014C

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