Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) POL2A Polyclonal antibody

Uniprot No.

F4HW04

Target Names

POL2A

Alternative Names

POL2A antibody; ABO4 antibody; EMB142 antibody; EMB2284 antibody; EMB529 antibody; ESD7 antibody; TIL1 antibody; At1g08260 antibody; T23G18.21DNA polymerase epsilon catalytic subunit A antibody; EC 2.7.7.7 antibody; DNA polymerase 2 a antibody; AtPOL2a antibody; DNA polymerase II subunit a antibody; Protein ABA OVERLY SENSITIVE a antibody; Protein EARLY IN SHORT DAYS 7 antibody; Protein EMBRYO DEFECTIVE 142 antibody; Protein EMBRYO DEFECTIVE 2284 antibody; Protein EMBRYO DEFECTIVE 529 antibody; Protein TILTED 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana POL2A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

DNA polymerase II, which participates in chromosomal DNA replication. Required for the timing and determination of cell fate during plant embryogenesis and root pole development, by promoting cell cycle and cell type patterning. Necessary for proper shoot (SAM) and root apical meristem (RAM) functions. Involved in maintaining epigenetic states, controlling hypersensitive response (HR), and mediating abscisic acid (ABA) signaling. Required for flowering repression through a mechanism involving epigenetic gene silencing. May participate in processes involved in chromatin-mediated cellular memory.

Gene References into Functions

Data indicate that mutations in DNA polymerase epsilon catalytic subunit A (POL2A) cause reduced fertility and meiotic defects, including abnormal chromosome association, improper chromosome segregation, and fragmentation. PMID: 26392549
The ESD7 gene encodes the catalytic subunit of DNA polymerase epsilon. The esd7-1 mutant is early flowering and displays pleiotropic defects in both vegetative and reproductive development. [ESD7] PMID: 19947980
POL2a is involved in maintaining epigenetic states, homologous recombination, and ABA signaling in Arabidopsis. PMID: 19244142

Subcellular Location

Nucleus.

Protein Families

DNA polymerase type-B family

Tissue Specificity

Mostly expressed at low levels in inflorescence (floral meristem and flowers until anthesis), and, to a lower extent, in roots, seeds and leaves.

Database Links

KEGG: ath:AT1G08260

STRING: 3702.AT1G08260.1

UniGene: At.42268

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POL2A Antibody

Product Details

Customer Reviews and Q&A

Target Background