43 Antibody

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Code	CSB-PA366150XA01EDZ
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) 43 Polyclonal antibody

Uniprot No.

P04415

Target Names

Alternative Names

43 antibody; DNA-directed DNA polymerase antibody; EC 2.7.7.7 antibody; EC 3.1.11.- antibody; Gene product 43 antibody; Gp43 antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage T4 (Bacteriophage T4)

Immunogen

Recombinant Enterobacteria phage T4 (Bacteriophage T4) 43 protein

Immunogen Species

Enterobacteria phage T4 (Bacteriophage T4)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Gene References into Functions

Amino acid substitutions that confer decreased ability to replicate DNA under low-dGTP conditions or drug sensitivity were identified in the new motif, which suggests that the motif functions in regulating the stability of polymerase complexes. PMID: 20493878
Generation of a gp43-gp59-DNA ternary complex is absolutely required for recombination-dependent replication in the bacteriophage T4 system. PMID: 15909989
the primer strand is not stably bound within the exonuclease active site in the absence of the beta hairpin loop PMID: 17098747
The authors propose that their beta loops facilitate strand separation, while the residues that form the loop have low structural constraints. PMID: 19409904

Protein Families

DNA polymerase type-B family

Database Links

KEGG: vg:1258685

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Service

Phage Display Service

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Protein Service

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43 Antibody

Product Details

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Target Background