REV3 Antibody

Code CSB-PA019577XA01SVG
Size Enquire
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REV3 Polyclonal antibody
Uniprot No.
Target Names
REV3
Alternative Names
REV3 antibody; PSO1 antibody; YPL167C antibody; P2535DNA polymerase zeta catalytic subunit antibody; EC 2.7.7.7 antibody; Protein reversionless 3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REV3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Translesion synthesis in S.cerevisiae may use a specialized DNA polymerase that is not required for other DNA replicative processes. Has a role in the bypass of abasic (AP) sites. Highly inefficient in incorporating nucleotides opposite the AP site, but efficiently extends from nucleotides, particularly an A, inserted opposite the lesion.
Gene References into Functions
  1. This study also uncovered an important role for Rev3 and Rev7 (but not for Pol32) subunits of polymerase zeta in the survival of cells undergoing telomere losses and forming palindromes PMID: 27334270
  2. A novel mutation affecting the catalytic subunit Rev3 and the role of Rev3 in the Pol32 regulation PMID: 24819597
  3. Strains deficient in Pol zeta (Rev3) show little bypass of the T-T (6-4) photoadduct or the abasic sites. PMID: 15520252
  4. a specific subset of the Pol zeta-dependent mutational events requires oxidative metabolism PMID: 16290107
  5. Physical association of Rev1 with DNA polymerase zeta is mediated through the carboxyl terminus of Rev1 and the polymerase domain of Rev3. PMID: 17030609
  6. error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiae PMID: 17396018
  7. Results imply that about 30% of starvation-associated base substitutions and frameshifts arise by some unknown mechanism that does not involve Pol zeta or NHEJ. PMID: 19305999
  8. mismatch repair system promotes Polzeta-dependent mutagenesis by inhibiting an alternative, error-free pathway that depends on homologous recombination PMID: 19307574
  9. The L979F Pol zeta, and perhaps wild-type Pol zeta, which also generates clustered mutations at a lower but significant rate, performs short patches of processive, error-prone DNA synthesis. PMID: 19380376

Show More

Hide All

Subcellular Location
Mitochondrion. Nucleus.
Protein Families
DNA polymerase type-B family
Database Links

KEGG: sce:YPL167C

STRING: 4932.YPL167C

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1