dpy-21 Antibody

Code CSB-PA946873XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans dpy-21 Polyclonal antibody
Uniprot No.
Target Names
dpy-21
Alternative Names
dpy-21 antibody; Y59A8B.1Lysine-specific demethylase 9 antibody; KDM9 antibody; EC 1.14.11.- antibody; Dosage compensation protein dpy-21 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans dpy-21 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone demethylase that specifically demethylates dimethylated 'Lys-20' of histone H4 (H4K20me2), thereby modulating the chromosome architecture. Promotes chromatin compaction by converting H4k20me2 to H4K20me1 leading to transcriptional repression. Required for X chromosome dosage compensation by enriching H4K20me1 on X chromosomes and thereby reducing X-linked gene transcription in hermaphrodites throughout development. X chromosome specificity is mediated by the recruitment through proteins of the condensin-like dosage compensation complex (DCC). Required for the enrichment of H4K20me1 on autosomes in meiotic germ cells leading to their compaction in a DCC-independent mechanism. Involved in 3-dimensional chromosome organization by strengthening the borders of topologically associating domains. Involved in the regulation of growth, fecundity and body fat metabolism downstream of the TOR complex 2 and the protein kinase sgk-1 pathway. Also involved in male tail development.
Gene References into Functions
  1. The structure and the activity of the dosage compensation complex (DCC) subunit DPY-21 define a Jumonji demethylase subfamily that converts H4K20me2 to H4K20me1 in worms and mammals; Unexpectedly, DPY-21 also associates with autosomes of germ cells in a DCC-independent manner to enrich H4K20me1 and trigger chromosome compaction. PMID: 28867287
  2. data suggest non-canonical, negative regulation of growth and reproduction by DPY-21 via DCC, SET-1 and SET-4 downstream of TORC2 in C. elegans. PMID: 23884442
Subcellular Location
Nucleus. Chromosome.
Protein Families
Round spermatid basic protein 1 family
Database Links

KEGG: cel:CELE_Y59A8B.1

STRING: 6239.Y59A8B.1a

UniGene: Cel.1621

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