Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DRB1 Polyclonal antibody

Uniprot No.

O04492

Target Names

DRB1

Alternative Names

DRB1 antibody; HYL1 antibody; At1g09700 antibody; F21M12.9Double-stranded RNA-binding protein 1 antibody; Protein HYPONASTIC LEAVES 1 antibody; dsRNA-binding protein 1 antibody; AtDRB1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DRB1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Double-stranded RNA-binding protein involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis by assisting DICER-LIKE 1 (DCL1) in the accurate processing from primary miRNAs (pri-miRNAs) to miRNAs in the nucleus. Forms a complex with SERRATE (SE) and DCL1 to promote accurate processing of pri-miRNAs by DCL1. Binds and assist DCL1 for accurate processing of precursor miRNAs (pre-miRNA). Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved with argonaute 1 (AGO1) in the guide strand selection from miRNA duplexes, presumably by directional loading of the miRNA duplex (guide stand and passenger strand) onto the RNA-induced silencing complex (RISC) for passenger strand degradation. Does not participate in sense transgene-induced post-transcriptional gene silencing (S-PTGS). Involved in several plant development aspects and response to hormones through its role in miRNAs processing.

Gene References into Functions

HYL1 negatively regulate plant photomorphogenesis. PMID: 29522510
High DRB1 expression is associated with defense against turnip crinkle virus. PMID: 29513740
KETCH1 transports HYL1 from the cytoplasm to the nucleus. PMID: 28348234
SMEK1, as a suppressor, inhibits MAPK activation to attenuate HYL1 phosphorylation; SMEK1 assembles a functional PP4 to target HYL1 for dephosphorylation PMID: 28586645
Enzymes of the fatty acid degradation pathway were upregulated in DRB1-mutated plants. PMID: 26387911
Data establishes that RNA binding by HYL1 dsRBD1 is essential for its function. PMID: 25409478
The results suggest that HYL1 ensures the correct selection of pri-miRNA cleavage sites through homodimerization and thus contributes to gene silencing and plant development. PMID: 25294831
The C-terminal dsRBDs of DCL1 and N-terminal dsRBDs of HYL1 have parallel roles. PMID: 23886622
The role of HYL1 is in establishment of stamen architecture provides insight into the molecular mechanism of male fertility. PMID: 23918970
HYL1 controls the miR156-mediated juvenile phase of vegetative growth. PMID: 22268150
HYL1 monitors the roles of miR165/166, miR319a, and miR160 in leaf flattening through the relative activities of adaxial and abaxial identity genes. PMID: 21610018
HYL1 has a function in assisting efficient and precise cleavage of pri-miRNA through interaction with DCL1 PMID: 16428603
The N-terminal double-stranded RNA binding domains fulfill the function of the whole HYL1 and thus play an essential role in miRNA processing and miRNA-directed silencing of targeted genes. PMID: 17337628
the distinct HYL1- and DCL1-containing nuclear bodies may be miRNA precursor processing sites or assembly and storage sites for the miRNA precursor processing machinery PMID: 17369351
SINE RNA interacts with HYL1 that associates with the Dicer homologue DCL1 to produce microRNAs. PMID: 18551175
Genetic screening identified a novel semidominant mutation in DCL1 (dcl1-13), this mutation confers significant restoration from the developmental abnormality and a reduction in the level of miRNA in the loss-of-function mutant of HYL1. PMID: 19155326
In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced pri-miRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs. PMID: 19304749
suggests that small RNA processing in the DCL1/DRB1-directed miRNA and DCL4/DRB4-directed sRNA biogenesis pathways operates via different mechanisms. PMID: 19861421

Hide All

Subcellular Location

Nucleus. Nucleus speckle. Note=Localizes to nuclear dicing body (also named D body), a nuclear body distributed throughout the nucleoplasm and involved in miRNA processing.

Tissue Specificity

Expressed in rosette and cauline leaves, stems, roots, flowers and siliques.

Database Links

KEGG: ath:AT1G09700

STRING: 3702.AT1G09700.1

UniGene: At.10223

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA520798XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

DRB1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background