Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DBR4 Polyclonal antibody

Uniprot No.

Q8H1D4

Target Names

DBR4

Alternative Names

DBR4 antibody; At3g62800 antibody; F26K9.230Double-stranded RNA-binding protein 4 antibody; dsRNA-binding protein 4 antibody; AtDRB4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DBR4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Double-stranded RNA-binding protein involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the trans-acting small interfering RNAs (ta-siRNAs) biogenesis by binding and assisting DICER-LIKE 4 (DCL4). Required for DCL4 activity. Required for the 21 nucleotide ta-siRNAs production of the TAS3 transcript in leaves but not in flowers. Plays an important role in silencing RNA of both DNA and RNA viruses. Involved with argonaute 7 (AGO7) and RDR6 in turnip crinkle virus (TCV) silencing. May not be directly involved in viral siRNA production. May stabilize the 21 nucleotide viral siRNAs and deliver them to the RISC complex. Targeted by the viral silencing suppressor (VSR) transactivator/viroplasmin (TAV) protein of the cauliflower mosaic virus (CaMV) that inactivates DRB4 function in RNA silencing. Probably not involved in the guide strand selection from RNA duplexes. Involved in leaf morphology through its function in ta-siRNA-mediated silencing.

Gene References into Functions

High DRB4 expression is associated with defense against turnip crinkle virus. PMID: 29513740
Data indicate a potential role of the C-terminal region of double-stranded-RNA-binding protein 4 (DRB4) in protein:protein interaction as it possesses six PxxP motifs, binds to Zn2+ and contains a small structural domain. PMID: 28575480
The DRB7.1-DRB4 complex can antagonize RNase III activity and the production of siRNAs in plants. PMID: 28232389
Turnip crinkle virus infection promotes the cytosolic accumulation of the otherwise primarily nuclear DRB4, and this in turn inhibits HRT-DRB4 interaction. PMID: 24614040
DCL4 and its interacting partner dsRNA-binding protein 4 (DRB4) participate in the antiviral response to Turnip yellow mosaic virus (TYMV), and that both proteins are required for TYMV-derived small RNA production. PMID: 21883552
DRB4 is specifically required for DCL4 activity in vitro. dsRNA-binding domains are critical for DRB4 function. PMID: 21270136
a fraction of P6 immunoprecipitates with DRB4 in CaMV-infected cells (viral P6). PMID: 18615098
implicate DRB4 in antiviral RNA silencing PMID: 18799732

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in roots, leaf vasculature, shoot apical meristem (SAM) and developing anthers.

Database Links

KEGG: ath:AT3G62800

STRING: 3702.AT3G62800.1

UniGene: At.34049

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DBR4 Antibody

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