Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) drk Polyclonal antibody

Uniprot No.

Q08012

Target Names

drk

Alternative Names

drk antibody; E(sev)2B antibody; CG6033 antibody; Protein enhancer of sevenless 2B antibody; Protein E(sev)2B antibody; Downstream of receptor kinase antibody; GRB2-related adapter protein homolog antibody; SH2-SH3 adapter protein drk antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) drk protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Adapter protein which modulates signaling mediated by several receptor tyrosine kinases such as sev and Ack. Required for proper signaling by sevenless. May act to stimulate the ability of Sos to catalyze Ras1 activation by linking sevenless and Sos in a signaling complex. Required for functional and morphological integrities of the scolopidia, sensory neurons and the antennal mechanosensory and motor center (AMMC) brain neuropil. Required for Ack-dependent suppression of apoptosis in the eye.

Gene References into Functions

Drk plays a negative role in EGFR signaling by enhancing receptor endocytosis through cooperating with the RING domain of D-Cbl. PMID: 21340027
The drkN SH3 domain exists in equilibrium between folded (F(exch)) and unfolded (U(exch)) states PMID: 11707108
size distribution of molecules within an unfolded state of the N-terminal SH3 domain of drk (drkN SH3) has been studied by small-angle X-ray scattering (SAXS) and pulsed-field-gradient NMR (PFG-NMR) methods PMID: 11829506
SH3 domain-mediated binding of the Drk protein to Dos is an important step in signaling of Drosophila receptor tyrosine kinases. PMID: 12128212
presence of residual structure in the unfolded state of the N-terminal SH3 domain of Drosophila drk (drkN SH3 domain) has been investigated using far- and near-UV circular dichroism (CD), fluorescence, and NMR spectroscopy PMID: 12215422
residue-specific pK(a) values for a thermodynamically unstable Src homology 3 domain, enabling the calculation of the pH dependence of stability based on simple analytical expressions PMID: 12671071
Structural characterizations of the drkN SH3 domain unfolded state. PMID: 17320108

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Subcellular Location

Membrane; Peripheral membrane protein. Cell junction, synapse.

Protein Families

GRB2/sem-5/DRK family

Tissue Specificity

Found mainly in the developing eye and in the antennal disk. Also observed in other imaginal disks tested and in the embryo. Expressed in Johnston's organ, the hearing organ, neurons (at protein level).

Database Links

KEGG: dme:Dmel_CG6033

STRING: 7227.FBpp0086812

UniGene: Dm.3707

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drk Antibody

Product Details

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