Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) dsbD Polyclonal antibody

Uniprot No.

P36655

Target Names

dsbD

Alternative Names

dsbD antibody; cutA2 antibody; cycZ antibody; dipZ antibody; b4136 antibody; JW5734Thiol:disulfide interchange protein DsbD antibody; EC 1.8.1.8 antibody; C-type cytochrome biogenesis protein CycZ antibody; Inner membrane copper tolerance protein antibody; Protein-disulfide reductase antibody; Disulfide reductase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) dsbD protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm, thereby maintaining the active site of DsbC, DsbE and DsbG in a reduced state. This transfer involves a cascade of disulfide bond formation and reduction steps.

Gene References into Functions

The authors show that the F plasmid-encoded TrbB relies on DsbD from Escherichia coli for maintenance of its C-X-X-C redox active site motif. PMID: 21742866
Cys109 and Cys461 form a transient interdomain disulfide bond between nDsbD and cDsbD in the reaction cycle of DsbD PMID: 15057279
kinetic analysis of DsbD-dependent cytochrome c maturation PMID: 16004871
N-terminal (nDsbD) and a C-terminal (cDsbD) are likely to be correlated to the role of cDsbD as a covalently linked electron shuttle between the membrane domain and the N-terminal periplasmic domain of DsbD PMID: 16545842
The structural features enabling nDsbD, a 'redox hub' with an immunoglobulin-like fold, to interact efficiently with its different thioredoxin-like partners are reviewed [review] PMID: 16786221
Properties of the mutants to the positions of the amino acids in the structure of DsbD and discuss mechanisms that would interfere with the electron transfer process. PMID: 16816179
Here we provide evidence that DsbD's conformation depends on its oxidation state. PMID: 17015672
pK(a) value of the second cysteine in wild-type cDsbD, Cys464, is significantly higher than the maximum pH value that was studied PMID: 17544440
analysis of the structure and function of DsbD PMID: 19258316

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Thioredoxin family, DsbD subfamily

Database Links

KEGG: ecj:JW5734

STRING: 316385.ECDH10B_4329

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dsbD Antibody

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