Name: dsbD Antibody
Brand: CUSABIO
SKU: CSB-PA327569XA01ENV

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) dsbD Polyclonal antibody

Uniprot No.

P36655

Target Names

dsbD

Alternative Names

dsbD antibody; cutA2 antibody; cycZ antibody; dipZ antibody; b4136 antibody; JW5734Thiol:disulfide interchange protein DsbD antibody; EC 1.8.1.8 antibody; C-type cytochrome biogenesis protein CycZ antibody; Inner membrane copper tolerance protein antibody; Protein-disulfide reductase antibody; Disulfide reductase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) dsbD protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm, thereby maintaining the active site of DsbC, DsbE and DsbG in a reduced state. This transfer involves a cascade of disulfide bond formation and reduction steps.

Gene References into Functions

The authors show that the F plasmid-encoded TrbB relies on DsbD from Escherichia coli for maintenance of its C-X-X-C redox active site motif. PMID: 21742866
Cys109 and Cys461 form a transient interdomain disulfide bond between nDsbD and cDsbD in the reaction cycle of DsbD PMID: 15057279
kinetic analysis of DsbD-dependent cytochrome c maturation PMID: 16004871
N-terminal (nDsbD) and a C-terminal (cDsbD) are likely to be correlated to the role of cDsbD as a covalently linked electron shuttle between the membrane domain and the N-terminal periplasmic domain of DsbD PMID: 16545842
The structural features enabling nDsbD, a 'redox hub' with an immunoglobulin-like fold, to interact efficiently with its different thioredoxin-like partners are reviewed [review] PMID: 16786221
Properties of the mutants to the positions of the amino acids in the structure of DsbD and discuss mechanisms that would interfere with the electron transfer process. PMID: 16816179
Here we provide evidence that DsbD's conformation depends on its oxidation state. PMID: 17015672
pK(a) value of the second cysteine in wild-type cDsbD, Cys464, is significantly higher than the maximum pH value that was studied PMID: 17544440
analysis of the structure and function of DsbD PMID: 19258316

Hide All

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Thioredoxin family, DsbD subfamily

Database Links

KEGG: ecj:JW5734

STRING: 316385.ECDH10B_4329

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dsbD Antibody

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