DSL1 Antibody

Code CSB-PA346892XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DSL1 Polyclonal antibody
Uniprot No.
Target Names
DSL1
Alternative Names
DSL1 antibody; YNL258C antibody; N0842Protein transport protein DSL1 antibody; Dependent on SLY1-20 protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DSL1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.
Gene References into Functions
  1. SNARE-mediated homotypic ER fusion requires DSL1. PMID: 25187651
  2. subunits of the Dsl1 complex (Dsl1, Dsl3, and Tip20), as well as the SNAREs Ufe1 and Sec20, are ER-resident proteins that do not seem to move into COPII vesicles. PMID: 21550981
  3. a direct role of the Dsl1 complex, in particular Tip20p, in the formation and stabilization of ER SNARE complexes. PMID: 21482823
  4. Studies indicate that Dsl1 tethering complex seems to be designed to expose an unstructured domain of Dsl1p at its top, which is required to capture vesicles. PMID: 20226673
  5. DSL1 is required for the stable interaction of the SNARE Use1p with a central subcomplex consisting of Tip20p and the SNARE proteins Ufe1p and Sec20p. PMID: 15958492
  6. SEC20, SEC39, and DSL1 are essential secretory genes involved in the early stages of peroxisome assembly PMID: 19346454
  7. Study determined the structure of the Dsl1 complex, revealing a tower containing at its base the binding sites for two endoplasmic reticulum SNAREs and at its tip a flexible lasso for capturing vesicles. PMID: 20005805

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Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein.
Database Links

KEGG: sce:YNL258C

STRING: 4932.YNL258C

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