DSP4 Antibody

Code CSB-PA559979XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DSP4 Polyclonal antibody
Uniprot No.
Target Names
DSP4
Alternative Names
DSP4 antibody; PTPKIS1 antibody; SEX4 antibody; At3g52180 antibody; F4F15.290Phosphoglucan phosphatase DSP4 antibody; chloroplastic antibody; EC 3.1.3.- antibody; AtPTPKIS1 antibody; Dual specificity protein phosphatase 4 antibody; Protein STARCH-EXCESS 4 antibody; AtSEX4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DSP4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Starch granule-associated phosphoglucan phosphatase involved in the control of starch accumulation. Acts as major regulator of the initial steps of starch degradation at the granule surface. Functions during the day by dephosphorylating the night-accumulated phospho-oligosaccharides. Can release phosphate from both the C6 and the C3 positions, but dephosphorylates preferentially the C6 position.
Gene References into Functions
  1. structural analysis of SEX4 bound to maltoheptaose and phosphate PMID: 24799671
  2. Data indicate that mutation of Cys130 renders SEX4 more sensitive to oxidative inactivation and less responsive to reductive reactivation. PMID: 22372537
  3. Results reveal the unique tertiary architecture of SEX4 that provides the physical basis for its function as a glucan phosphatase. PMID: 20679247
  4. At3g52180 binds to purified starch through its predicted carbohydrate-binding region, and mutation of key conserved residues reduces this binding. PMID: 16623901
  5. DSP4 acts as a bridge between light-induced redox changes and protein phosphorylation in the regulation of starch accumulation [DSP4] PMID: 16772378
  6. SEX4 is a phosphoglucan phosphatase which dephosphorylates both the starch granule surface and soluble phosphoglucans. PMID: 19141707

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Subcellular Location
Plastid, chloroplast. Note=Associated with starch granule.
Database Links

KEGG: ath:AT3G52180

STRING: 3702.AT3G52180.1

UniGene: At.24067

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