dx Antibody

Code CSB-PA631531XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) dx Polyclonal antibody
Uniprot No.
Target Names
dx
Alternative Names
dx antibody; CG3929Protein deltex antibody; Probable E3 ubiquitin-protein ligase deltex antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase deltex antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) dx protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it may also act as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch. May function as a ubiquitin ligase protein in the Notch pathway.
Gene References into Functions
  1. we observed that Dx together with Hrp48 can regulate Notch signaling in an Sxl-independent manner. In addition, Dx and Hrp48 displayed a synergistic effect on caspase-mediated cell death. Our results suggest that Dx and Hrp48 together negatively regulate Notch signaling in Drosophila melanogaster. PMID: 28396507
  2. oligomerization mediated by the RING-H2 finger motif is an integral step in the signaling function of Deltex.(regulation of Notch signaling) PMID: 11861487
  3. the accumulation of Notch (N) in the late-endosome was required for the Dx-mediated Su(H)-independent N signaling PMID: 15496440
Subcellular Location
Cytoplasm.
Protein Families
Deltex family
Tissue Specificity
Ubiquitous. Expressed at low levels throughout embryogenesis and in larvae.
Database Links

KEGG: dme:Dmel_CG3929

STRING: 7227.FBpp0070876

UniGene: Dm.2626

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