DUN1 Antibody

Code CSB-PA327922XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DUN1 Polyclonal antibody
Uniprot No.
Target Names
DUN1
Alternative Names
DUN1 antibody; YDL101C antibody; D2370DNA damage response protein kinase DUN1 antibody; EC 2.7.11.1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DUN1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transducer of the DNA damage signal. Phosphorylates SML1 on serine residues. Cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress.
Gene References into Functions
  1. Mitochondrial mutations occurring more frequently in yeast devoid of Rrm3, a DNA helicase involved in rDNA replication, are also dependent on Dun1. PMID: 28678842
  2. these results reveal that, in response to iron starvation, Dun1 kinase phosphorylates Dif1 to stimulate Rnr2-Rnr4 relocalization to the cytoplasm and promote RNR function. PMID: 26970775
  3. levels of the Sml1 protein specifically decrease in response to both nutritional and genetic Fe deficiencies in a Dun1-dependent but Mec1/Rad53- and Aft1-independent manner PMID: 24958100
  4. Multiple DNA damage checkpoint kinase Dun1 regulates the Asf1-Rad53 interaction and therefore affects the activity of the Asf1/Hir complex in vivo. PMID: 16020781
  5. Rad53 was found to be directly responsible for Dun1 activation. PMID: 17114794
  6. MEC1 (ATR), RAD53, and DUN1 have roles in stimulation of sister chromatid exchanges and mutation by aflatoxin B1-DNA adducts in Saccharomyces cerevisiae PMID: 18228255
  7. DIN7 repression by Dun1 can be released upon DNA damage PMID: 18540090
  8. Dun1-forkhead-associated domains possess an unprecedented diphosphothreonine-binding specificity. PMID: 18570878

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Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily
Database Links

KEGG: sce:YDL101C

STRING: 4932.YDL101C

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