DUO1 Antibody

Code CSB-PA934269XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DUO1 Polyclonal antibody
Uniprot No.
Target Names
DUO1
Alternative Names
DUO1 antibody; MYB125 antibody; At3g60460 antibody; T8B10.120Transcription factor DUO1 antibody; Myb-related protein 125 antibody; AtMYB125 antibody; Protein DUO POLLEN 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DUO1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression. Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization. Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon. Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression.
Gene References into Functions
  1. key promoter sequences required for the germline-specific regulation of DUO1 transcription, are identified. PMID: 27624837
  2. The DUO1 regulon has a major role in shaping the germline transcriptome and functions to commit progenitor germ cells to sperm cell differentiation. PMID: 21285328
  3. DUO1 controls male gamete formation in plants. [DUO1] PMID: 15694308
Subcellular Location
Nucleus.
Tissue Specificity
Confined to inflorescences, especially in stamens and pollen.
Database Links

KEGG: ath:AT3G60460

STRING: 3702.AT3G60460.1

UniGene: At.34443

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