gcn-2 Antibody

Code CSB-PA260418XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans gcn-2 Polyclonal antibody
Uniprot No.
Target Names
gcn-2
Alternative Names
gcn-2 antibody; Y81G3A.3Eukaryotic translation initiation factor 2-alpha kinase gcn-2 antibody; EC 2.7.11.1 antibody; General control nonderepressible kinase 2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans gcn-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase which phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Involved in the unfolded protein response (UPR) triggered by several stresses including mitochondrial, osmotic and oxidative stresses, amino acid deprivation and UV irradiation, probably by phosphorylating and inhibiting eIF2alpha. In addition, leads to the selective translation/transcription of some mRNA including atf-5, pha-4 and gpdh-1 which are part of the UPR. Required for maintaining lifespan during amino acid starvation. Involved in hypoxia-mediated adaptive protective response.
Gene References into Functions
  1. GCN-2 dependent inhibition of protein synthesis activates osmosensitive gene transcription via WNK and Ste20 kinase signaling PMID: 23076791
  2. The experiments described here implicate the eIF2alpha kinase GCN-2 in the maintenance of mitochondrial function and protein homeostasis. PMID: 22719267
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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