E2FC Antibody

Code CSB-PA863845XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) E2FC Polyclonal antibody
Uniprot No.
Target Names
E2FC
Alternative Names
E2FC antibody; E2F2 antibody; At1g47870 antibody; T2E6.2Transcription factor E2FC antibody; E2F transcription factor-2 antibody; AtE2F2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana E2FC protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Involved in transcriptional repression. May act by repressing E2F-regulated genes in mature differentiated cells, but is not an antagonist of E2FA. Restricts cell division and is involved in the coordination between cell proliferation and endoreduplication during development. May play a role during the transition from skotomorphogenesis to photomorphogenesis. Regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(SKP2A) complex.
Gene References into Functions
  1. E2Fc induction led primarily to gene downregulation, and appears to function outside the CYCD3-RBR pathway, does not have a direct effect on cell cycle genes, and promoter analysis suggests a distinct binding site preference. PMID: 19662336
Subcellular Location
Cytoplasm. Note=Interaction with either DPA or DPB are unable to induce a nuclear localization.
Protein Families
E2F/DP family
Tissue Specificity
Expressed in meristematic areas, vascular tissues, apical part of the roots, cotyledons, upper region of the hypocotyls, trichomes, young flower buds and pollen grains.
Database Links

KEGG: ath:AT1G47870

STRING: 3702.AT1G47870.1

UniGene: At.10547

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