E2FE Antibody

Code CSB-PA839710XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) E2FE Polyclonal antibody
Uniprot No.
Target Names
E2FE
Alternative Names
E2FE antibody; DEL1 antibody; E2L3 antibody; ELP2 antibody; At3g48160 antibody; T24C20.40 antibody; E2F transcription factor-like E2FE antibody; DP-E2F-like protein 1 antibody; E2F-like repressor E2L3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana E2FE protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Inhibitor of E2F-dependent activation of gene expression. Binds specifically the E2 recognition site without interacting with DP proteins and prevents transcription activation by E2F/DP heterodimers. Controls the timing of endocycle onset and inhibits endoreduplication.
Gene References into Functions
  1. Del1 Knockout Mice Developed More Severe Osteoarthritis Associated with Increased Susceptibility of Chondrocytes to Apoptosis PMID: 27505251
  2. involved in nematode feeding site generation PMID: 23518580
  3. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. PMID: 22640471
  4. Overexpression of DEL1 does not completely abolish early endoreduplication on stress. PMID: 22535421
  5. The Arabidopsis (Arabidopsis thaliana) DEL1 gene was identified as a transcriptional target of the classical E2Fb and E2Fc transcription factors. PMID: 21908689
  6. DEL1 is a transcriptional repressor of the type-II cyclobutane pyrimidine dimer-photolyase DNA repair gene PHR1. PMID: 21131907
  7. DEL1 (EL3) is an important novel inhibitor of the endocycle and preserves the mitotic state of proliferating cells by suppressing transcription of genes that are required for cells to enter the DNA endoreduplication cycle. PMID: 15649366
  8. the atypical E2F transcription factor E2Fe/DEL1 controls the expression of the CDH1/FZR orthologous CCS52A2 gene from Arabidopsis thaliana PMID: 18787127

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Subcellular Location
Nucleus.
Protein Families
E2F/DP family
Tissue Specificity
Expressed exclusively in mitotically dividing cells. Highly expressed in young leaves and mature flowers. Lower expression in young stalk and in young and mature flowers.
Database Links

KEGG: ath:AT3G48160

STRING: 3702.AT3G48160.2

UniGene: At.35736

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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