E2FF Antibody

Code CSB-PA837283XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) E2FF Polyclonal antibody
Uniprot No.
Target Names
E2FF
Alternative Names
E2FF antibody; DEL3 antibody; E2L2 antibody; ELP1 antibody; At3g01330 antibody; T22N4.4E2F transcription factor-like E2FF antibody; DP-E2F-like protein 3 antibody; E2F-like repressor E2L2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana E2FF protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Inhibitor of E2F-dependent activation of gene expression. Binds specifically the E2 recognition site without interacting with DP proteins and prevents transcription activation by E2F/DP heterodimers. Does not bind retinoblastoma-related proteins. Acts as a growth regulator but is not associated with changes in the expression of cell cycle marker genes or in nuclear ploidy levels. Has no effect on cell proliferation, but may repress cell wall biosynthesis genes during cell elongation in differentiated cells.
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
E2F/DP family
Tissue Specificity
High expression in young cotyledons and leaves, hypocotyls, shoot apical meristem, roots and mature pollen grains, moderate in developing trichomes, flowers and at early stages of developing anthers, and barely detectable in mature leaves. Not detected in
Database Links

KEGG: ath:AT3G01330

STRING: 3702.AT3G01330.1

UniGene: At.41295

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