Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EXO70A1 Polyclonal antibody

Uniprot No.

Q9LZD3

Target Names

EXO70A1

Alternative Names

EXO70A1 antibody; At5g03540 antibody; F12E4.320Exocyst complex component EXO70A1 antibody; AtExo70a1 antibody; Exocyst subunit Exo70 family protein A1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EXO70A1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane during regulated or polarized secretion. Involved in polarized cell growth and organ morphogenesis. Involved in polarized cell growth and organ morphogenesis. During cytokinesis, involved in cell plate initiation, cell plate maturation and formation of new primary cell wall. Participates in polarized pectin delivery required for the polarized development of the mucilage-producing volcano cells of the seed coat. Involved in the recycling and localization of auxin efflux carriers PIN1 and PIN2, and thus in polar auxin transport regulation. Functions in vesicle trafficking in tracheary elements to regulate patterned secondary cell wall (SCW) thickening.

Gene References into Functions

The authors' results establish EXO70A1 as a central player in Casparian strip formation, generating a transient positional information that will be translated into a precisely localized cell wall modification. PMID: 28436943
Arabidopsis EXO70A1 recruits Patellin3 to the cell membrane independent of its role as an exocyst subunit PMID: 28815958
small molecule Endosidin2 (ES2) binds to the EXO70 (exocyst component of 70 kDa) subunit of the exocyst complex, resulting in inhibition of exocytosis and endosomal recycling in both plant and human cells and enhancement of plant vacuolar trafficking. PMID: 26607451
Data suggest that EXO70A1 functions in vesicle trafficking in tracheary element (TE) to regulate patterned secondary cell wall thickening. PMID: 23709627
study implies that multiplications of EXO70 genes may allow plants to acquire cell type- and/or cargo-specific regulatory machinery for exocytosis PMID: 20943851
Exo70A1 is a compatibility factor in the stigma. Arabidopsis lines lacking Exo70A1 in the stigma are incapable of accepting compatible pollen. PMID: 19789280
The putative exocyst subunit EXO70A1 is involved in cell and organ morphogenesis. [EXO70A1] PMID: 16942608
EXO70A1 copurifies in a high molecular mass fraction of 900 kD, interacts with SEC3a, and functions as a subunit in a exocyst complex that plays important roles in morphogenesis. PMID: 18492870

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Subcellular Location

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, phragmoplast. Cell membrane. Secreted, cell wall.

Protein Families

EXO70 family

Database Links

KEGG: ath:AT5G03540

STRING: 3702.AT5G03540.3

UniGene: At.4876

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EXO70A1 Antibody

Product Details

Customer Reviews and Q&A

Target Background