EB1C Antibody

Code CSB-PA583907XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EB1C Polyclonal antibody
Uniprot No.
Target Names
EB1C
Alternative Names
EB1C antibody; At5g67270 antibody; K3G17.3Microtubule-associated protein RP/EB family member 1C antibody; APC-binding protein EB1C antibody; End-binding protein 1C antibody; AtEB1C antibody; Protein ATEB1 homolog 1 antibody; AtEB1H1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EB1C protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plant-specific EB1 subtype that functions preferentially at early stages of plant mitosis by regulating spindle positioning and chromosome segregation. Accumulates in the prophase nucleus and is required to maintain spindle bipolarity during premetaphase and/or metaphase and for efficient segregation of chromosomes at anaphase. May play a role in the dynamics of microtubule network in elongating pollen tubes.
Gene References into Functions
  1. Data find that antibodies directed against EB1C proteins colocalize with microtubules in roots, and that mutants with reduced expression from EB1 genes have roots that deviate toward the left on vertical or inclined plates. PMID: 18281505
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=During mitosis, accumulates in the prophase nucleus, and after the nuclear envelope disintegration is associated with whole spindle microtubules, plus end of microtubules, phragmoplast and finally is actively recruited to the nucleus. Localizes in the microtubule network in elongating pollen tubes.
Protein Families
MAPRE family
Tissue Specificity
Highly expressed in the root and shoot meristems, in guard cells of leaf stomata, pollen grains and pollen tubes.
Database Links

KEGG: ath:AT5G67270

STRING: 3702.AT5G67270.1

UniGene: At.28817

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