ECM29 Antibody

Code CSB-PA336485XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ECM29 Polyclonal antibody
Uniprot No.
Target Names
ECM29
Alternative Names
ECM29 antibody; YHL030WProteasome component ECM29 antibody; Extracellular mutant protein 29 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ECM29 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Stabilizes the proteasome holoenzyme, probably by tethering the 20S proteolytic core particle and the 19S regulatory particle. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Gene References into Functions
  1. Ecm29 requires the phosphorylated tail of the proteasome subunit alpha7 for its association with proteasomes. PMID: 27302526
  2. Data show that Ecm29 acts as a proteasome inhibitor. PMID: 23995839
  3. results characterize Ecm29 as a proteasome chaperone whose appropriate interaction with the proteasome requires Not4 PMID: 21321079
  4. Studies indicate that deletion of ECM29 prevented the disassembly of the 26S proteasome in response to oxidative stress, and ecm29 mutants were more sensitive to HO than were wild-type cells. PMID: 21139140
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
ECM29 family
Database Links

KEGG: sce:YHL030W

STRING: 4932.YHL030W

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