tufA Antibody

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.; May play an important regulatory role in cell growth and in the bacterial response to nutrient deprivation.; Plays a stimulatory role in trans-translation; binds tmRNA.; Protects glycyl-tRNA(Gly) from hydrolysis by E.coli D-aminoacyl-tRNA deacylase (dtd).; (Microbial infection) Upon infection by bacteriophage Qbeta, part of the viral RNA-dependent RNA polymerase complex. With EF-Ts may provide a stabilizing scaffold for the beta (catalytic) subunit. Helps separate the double-stranded RNA of the template and growing RNA during elongation. With the beta subunit helps form the exit tunnel for template RNA.; (Microbial infection) Required for the tRNase activity of CdiA-CT from E.coli strain EC869; the toxic CT module is thought to cleave tRNA in the context of translationally active GTP EF-Tu-aa-tRNA complexes. GTP is required for tRNase activity but is not hydrolyzed. CdiA-CT is the toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. EF-Tu interacts with at least 2 different toxic CT domains, the 2 toxins are different and degrade tRNA at different positions.; (Microbial infection) Required for the tRNase activity of CdiA-CT from E.coli strain NC101; the toxic CT module is thought to cleave tRNA in the context of translationally active GTP EF-Tu-aa-tRNA complexes. The toxin may remodel the EF-Tu-aa-tRNA complex to displace the 3'-end of the aa-tRNA so it can enter the toxin active site and be cleaved. GTP is required for tRNase activity but is not hydrolyzed. CdiA-CT is the toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. EF-Tu interacts with at least 2 different toxic CT domains, the 2 toxins are different and degrade tRNA at different positions.