Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans egl-9 Polyclonal antibody

Uniprot No.

G5EBV0

Target Names

egl-9

Alternative Names

egl-9 antibody; F22E12.4Hypoxia-inducible factor prolyl hydroxylase antibody; HIF-PH antibody; EC 1.14.11.29 antibody; Egg-laying defective protein 9 antibody; Hypoxia-inducible factor-proline dioxygenase antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans egl-9 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Cellular oxygen sensor which regulates the stability and the activity of hypoxia-inducible transcription factor, hif-1. In normoxic conditions, hydroxylates hif-1 targeting it for vhl-1-mediated proteasomal degradation. In addition, regulates hif-1 transcriptional activity in a vhl-1-independent manner and independently of its hydroxylase activity. By regulating hif-1 activity, controls several cellular responses. Mediates susceptibility to B.thuringiensis and V.cholerae pore-forming toxins and enteropathogenic E.coli. Mediates susceptibility to P.aeruginosa PAO1-mediated killing by regulating resistance to cyanide produced by P.aeruginosa. Mediates resistance to S.aureus-mediated killing. In addition, plays a role in heat acclimation, neuronal development, behavioral responses to reoxygenation and hydrogen sulfide, iron homeostasis and aging. In neurons, involved in mitochondrion fusion during reoxygenation. Involved in egg laying.; Regulates the trafficking of the glutamate receptor glr-1, probably independently of hif-1, by regulating lin-10 subcellular localization in response to oxygen levels. May hydroxylate lin-10.

Gene References into Functions

study demonstrated that loss of egl-9, a negative regulator of HIF, confers HIF-dependent enhanced susceptibility to S. aureus while increasing resistance to Pseudomonas aeruginosa PMID: 22792069
These data support a model in which SWAN-1, MBK-1 and EGL-9 regulate HIF-1 transcriptional activity and modulate C. elegans resistance to P. aeruginosa PAO1 fast killing. PMID: 20865124
Data show that EGL-9 inhibits HIF-1 transcriptional activity via a pathway that has little or no requirement for hydroxylase activity or for the EGL-9 MYND domain. PMID: 19737748

Subcellular Location

[Isoform a]: Cytoplasm. Nucleus. Cell projection, dendrite.; [Isoform c]: Cytoplasm. Nucleus. Cell projection, dendrite.; [Isoform e]: Cytoplasm. Nucleus. Cell projection, axon.

Tissue Specificity

In larvae and adults, expressed in pharyngeal and body wall muscles.

Database Links

KEGG: cel:CELE_F22E12.4

STRING: 6239.F22E12.4a.1

UniGene: Cel.18026

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egl-9 Antibody

Product Details

Customer Reviews and Q&A

Target Background