EGY1 Antibody

Code CSB-PA171921XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EGY1 Polyclonal antibody
Uniprot No.
Target Names
EGY1
Alternative Names
EGY1 antibody; AMOS1 antibody; At5g35220 antibody; T25C13.100Probable zinc metalloprotease EGY1 antibody; chloroplastic antibody; EC 3.4.24.- antibody; Protein AMMONIUM OVERLY SENSITIVE 1 antibody; Protein ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN 1 antibody; AtEGY1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EGY1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Membrane-associated and ATP-independent metalloprotease required for development of both thylakoid grana and well-organized lamellae in chloroplast. Required for the accumulation of chlorophyll and chlorophyll a/b binding (CAB) proteins (from both PS I and PS II) in chloroplast membranes, and for grana formation and normal chloroplast development. Involved in the regulation of nuclear gene expression in response to ammonium stress and interacts with ABA signaling. Carries out beta-casein degradation in an ATP-independent manner in vitro.
Gene References into Functions
  1. Loss of function of AMOS1/EGY1 showed resistance to phosphate (P) starvation and retarded root growth and reduced P accumulation in the root. PMID: 27516532
  2. An ammonium (NH(4)(+))-responsive plastid retrograde pathway, which depends on AMOS1/EGY1 function and integrates with abscissic acid signaling, is required for the regulation of expression of NH(4)(+)-responsive genes PMID: 23064408
  3. The isolation and expression of EGY1, which affects both chlorophyll accumulation and gravitropism in A. thaliana seedlings. [EGY1] PMID: 15659096
  4. Role of EGY1 in regulation of endodermal plastid size and number as well as the stimulatory effect of ethylene on hypocotyl gravitropism in Arabidopsis PMID: 18097640
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein. Note=Localizes in endodermal and cortex plastids.
Protein Families
Peptidase M50B family
Tissue Specificity
Expressed in roots, leaves, cotyledons, hypocotyls, stems, flowers and siliques.
Database Links

KEGG: ath:AT5G35220

STRING: 3702.AT5G35220.1

UniGene: At.26562

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