GCN3 Antibody

Code CSB-PA007514XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GCN3 Polyclonal antibody
Uniprot No.
Target Names
GCN3
Alternative Names
GCN3 antibody; AAS2 antibody; TIF221 antibody; YKR026CTranslation initiation factor eIF-2B subunit alpha antibody; GCD complex subunit GCN3 antibody; Guanine nucleotide exchange factor subunit GCN3 antibody; Transcriptional activator GCN3 antibody; eIF-2B GDP-GTP exchange factor subunit alpha antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GCN3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the translation of GCN4 in response to low amino acid, carbon, or purine availability, by suppressing the inhibitory effects of multiple uORFs present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally.
Gene References into Functions
  1. Maintenance of inhibition required GCN-dependent hyperphosphorylation of eIF2alpha PMID: 15930127
Protein Families
EIF-2B alpha/beta/delta subunits family
Database Links

KEGG: sce:YKR026C

STRING: 4932.YKR026C

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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