RPG1 Antibody

Code CSB-PA007543XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPG1 Polyclonal antibody
Uniprot No.
Target Names
RPG1
Alternative Names
RPG1 antibody; TIF32 antibody; YBR079C antibody; YBR0734Eukaryotic translation initiation factor 3 subunit A antibody; eIF3a antibody; Eukaryotic translation initiation factor 3 110 kDa subunit homolog antibody; eIF3 p110 antibody; Translation initiation factor eIF3 antibody; p110 subunit homolog antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPG1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
Gene References into Functions
  1. The interaction between the flexible RPS0A-C-terminal tail and residues 200-400 of the TIF32-N-terminal domain significantly stimulates attachment of eIF3 and its associated eIFs to small ribosomal subunits in vivo. PMID: 22792338
  2. The monoclonal antibody PK1 recognized eIFa, the mammalian orthologue of yeast Rpg1, in CHO cells. PMID: 15913035
  3. eIF3 is retained on ribosomes throughout uORF1 translation and, interacts with its 5' enhancer at the mRNA exit channel to stabilize mRNA association with post-termination 40S subunits and enable resumption of scanning for reinitiation downstream. PMID: 18765792
Subcellular Location
Cytoplasm.
Protein Families
EIF-3 subunit A family
Database Links

KEGG: sce:YBR079C

STRING: 4932.YBR079C

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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