eIF3e Antibody

Code CSB-PA007534XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) eIF3e Polyclonal antibody
Uniprot No.
Target Names
eIF3e
Alternative Names
eIF3e antibody; eIF-3p48 antibody; eIF3-S6 antibody; Int6 antibody; CG9677 antibody; Eukaryotic translation initiation factor 3 subunit E antibody; eIF3e antibody; Eukaryotic translation initiation factor 3 subunit 6 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) eIF3e protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In addition to its role in the eIF-3 complex, also functions in protein ubiquitination and degradation. During mitosis required for regulating mitotic microtubule growth and kinetochore formation, and consequently is required for satisfying the spindle assembly checkpoint (SAC) during metaphase to prevent delays in mitotic progression. This is likely by promoting the ubiquitination and degradation of Klp67A, a kinesin-like protein that suppresses microtubule polymerization at plus ends. Acts in the COP9 signalosome (CSN) mediated regulation of cullin neddylation by promoting Cul1 and Cul3 neddylation and negatively regulating the CSN complex subunit CSN5.
Gene References into Functions
  1. Depletion of Int6 results in short mitotic spindles and deformed centromeres and kinetochores. Int6-mediated ubiquitination is required to control the activity of Klp67A. PMID: 28505193
  2. Int6 and the COP9 signalosome play opposing roles in regulation of cullin neddylation PMID: 18493598
Subcellular Location
Cytoplasm. Microsome. Endoplasmic reticulum.
Protein Families
EIF-3 subunit E family
Tissue Specificity
Expression levels in females and males are relatively similar 10 days after oviposition, however by day 15 expression is higher in gravid females than in males (at protein level).
Database Links

KEGG: dme:Dmel_CG9677

STRING: 7227.FBpp0075104

UniGene: Dm.3105

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