apela Antibody

Code CSB-PA123870XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) apela Polyclonal antibody
Uniprot No.
Target Names
apela
Alternative Names
apela antibody; ela antibody; tdlApelin receptor early endogenous ligand antibody; Protein Elabela antibody; ELA antibody; Protein Toddler antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) apela protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Endogenous ligand for the apelin receptor (aplnra and/or aplnrb). Hormone required for mesendodermal differentiation, blood vessels formation and heart morphogenesis during early development and for adult cardiovascular homeostasis. Acts as a motogen by promoting mesendodermal cell migration during gastrulation by binding and activating the apelin receptor. Acts as an early embryonic regulator of cellular movement with a role in migration and development of cardiac progenitor cells. May act as a chemoattractant for the activation of angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis. Positively regulates sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel sprouting. Involved in cardioprotective functions during heart failure. Mediates myocardial contractility in an ERK1/2-dependent manner.
Gene References into Functions
  1. GPR25 is activated by Apela protein. PMID: 29727602
  2. Angioblasts rely on their intrinsic expression of Apelin receptors for their migration to the midline. PMID: 26017639
  3. o pojho o oj pj;kjjpj poj po po po ;pj PMID: 24924601
  4. These results indicate that Toddler is an activator of APJ/Apelin receptor signaling, promotes gastrulation movements, and might be the first in a series of uncharacterized developmental signals. PMID: 24407481
Subcellular Location
Secreted. Secreted, extracellular space.
Protein Families
Elabela/Toddler family
Tissue Specificity
Expressed ubiquitously during late blastula and gastrula stages and becomes restricted to the lateral mesoderm, endoderm, and anterior and posterior notochord after gastrulation.
Database Links

KEGG: dre:100536023

UniGene: Dr.81857

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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