ELG1 Antibody

Code CSB-PA606288XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ELG1 Polyclonal antibody
Uniprot No.
Target Names
ELG1
Alternative Names
ELG1 antibody; RTT110 antibody; YOR144C antibody; O3510 antibody; YOR3510CTelomere length regulation protein ELG1 antibody; Regulator of Ty1 transposition protein 110 antibody; Telomere elongation suppressor ELG1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ELG1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the negative control of telomere length and in telomeric silencing through a replication-mediated pathway. May have a role in Okazaki fragment maturation. Required for S-phase progression. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.
Gene References into Functions
  1. ELG1 deletion from pds5-1 mutant cells results in a significant rescue of chromatid cohesion, but not condensation, defects PMID: 25986377
  2. A high copy number suppressor screen was performed to search for genes that, when overexpressed, suppress the synthetic lethality between elg1Delta and srs2Delta mutants. PMID: 23704284
  3. Elg1 physically contacts the Mhf1/Mhf2 histone-like complex and genetically interacts with MPH1 (ortholog of the FANCM helicase) and CHL1 (ortholog of the FANCJ helicase) genes. PMID: 23624835
  4. Elg1 binds proteins that interact with SUMO. PMID: 21869594
  5. elg1-deficient Saccharomyces cerevisiae mutants have increased genome instability and telomere length regulated by S-phase checkpoints PMID: 15590829
  6. Elg1 function is required for maintaining genome stability during normal growth, and its absence has severe genetic consequences. PMID: 15725622
  7. Results suggest that Elg1 is involved in double strand break repair repair by homologous recombination. PMID: 17170004
  8. demonstrated that the N-terminus of Elg1 contributes to the maintenance of genome stability, and that one function of this N-terminus is to promote the nuclear localization of Elg1 PMID: 18482875
  9. Results suggest that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring. PMID: 19430531

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Subcellular Location
Nucleus. Chromosome, telomere.
Protein Families
ELG1 family
Database Links

KEGG: sce:YOR144C

STRING: 4932.YOR144C

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