Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HAG3 Polyclonal antibody

Uniprot No.

Q93ZR1

Target Names

HAG3

Alternative Names

HAG3 antibody; EAST1 antibody; ELO3 antibody; ELP3 antibody; At5g50320 antibody; MXI22.3Elongator complex protein 3 antibody; AtELP3 antibody; Elongator component 3 antibody; Protein ELONGATA 3 antibody; Protein ENHANCER-OF-ASYMMETRIC-LEAVES-TWO1 antibody; tRNA uridine(34) acetyltransferase antibody; EC 2.3.1.- antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HAG3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Involved in tRNA wobble uridine modification. In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Promotes organs development by modulating cell division rate. Required for auxin distribution or signaling. Required for meristem cell cycle activation at the time of germination. Mediates the establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway. Negatively regulates the expression of genes that participate in UV-B responses, such as DNA repair enzymes and enzymes that participate in sunscreen pigment biosynthesis.

Gene References into Functions

Data indicate that histone acetyltransferase HAG3, directly or indirectly, participates in UV-B-induced DNA damage repair and signaling. PMID: 25907565
ELONGATA3 (ELO3) as a key regulator of meristem cell cycle activation in Arabidopsis. PMID: 23969312
AtELP3 regulates plant immunity through its histone acetyltransferase and radical S-adenosylmethionine domains. PMID: 23856002
ELO3 mediated establishment of leaf polarity independently of AS2 and the trans-acting small interfering RNA-related pathway. PMID: 21700721
Results conclude that yeast and plant Elongator (elp3/ELO3) share tRNA modification roles and propose that this function might be conserved in Elongator from all eukaryotic kingdoms of life. PMID: 20398216

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

ELP3 family

Tissue Specificity

Expressed in meristematic tissues of in roots, leaves, seedlings, cotyledons, floral buds and shoot apices. Expressed in the shoot meristem and the emerging leaves immediately after germination. In the root, expressed in the transition zone and the upper

Database Links

KEGG: ath:AT5G50320

STRING: 3702.AT5G50320.1

UniGene: At.20657

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HAG3 Antibody

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