Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EMF1 Polyclonal antibody

Uniprot No.

Q9LYD9

Target Names

EMF1

Alternative Names

EMF1 antibody; At5g11530 antibody; F15N18.120Protein EMBRYONIC FLOWER 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EMF1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription repressor that regulates phase transition during shoot, flower and seeds development. Controls leaves development, shoot architecture and flowering by delaying both the vegetative to reproductive transition and flower initiation. Participates in polycomb group (PcG) protein complex-mediated (including EMF2) silencing of the flower homeotic genes AGAMOUS (AG), PISTILLATA (PI), and APETALA3 (AP3), as well as of some regulatory genes such as ABSCISIC ACID INSENSITIVE3 (ABI3), LONG VEGETATIVE PHASE1 (LOV1), and FLOWERING LOCUS C (FLC) during vegetative development. Required for histone methylation or for maintaining a stable histone methylation (e.g. H3K27me3) pattern of repressed target genes (including genes involved in salt stress response and flower development); this repression is counteracted by ULT1. Can bind non specifically DNA (both double- and single-stranded) and RNA.

Gene References into Functions

to promote vegetative development and to regulate cell differentiation during shoot and root organogenesis, EMF1 binds genes required for other developmental phases. These are primarily genes trimethylated by EMF2-PRC2 on their H3K27 PMID: 22457632
EMF1 interacts with EIP1, EIP6 and EIP9 during vegetative development to regulate flowering time in Arabidopsis. PMID: 21700722
EMF1 and EMF2 target transcription factor genes to mediate vegetative development and stress responses, and regulate flowering time genes. PMID: 19783648
Results are consistent with EMF1's primary role in repressing flowering in order to allow for vegetative growth. PMID: 19825645

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in mature embryo, root tips, cotyledons, leaves, stems, shoot apex, and flower clusters, with highest levels in flowers. The presence in the shoot apical meristem (SAM) is required to maintain vegetative development and prevent early flowering.

Database Links

KEGG: ath:AT5G11530

STRING: 3702.AT5G11530.1

UniGene: At.43396

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EMF1 Antibody

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