nth Antibody

Code CSB-PA447553XA01FSG
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Product Details

Full Product Name
Rabbit anti-Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) nth Polyclonal antibody
Uniprot No.
Target Names
nth
Alternative Names
nth antibody; Rv3674c antibody; MTV025.022cEndonuclease III antibody; EC 4.2.99.18 antibody; DNA-(apurinic or apyrimidinic site) lyase antibody
Raised in
Rabbit
Species Reactivity
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Immunogen
Recombinant Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) nth protein
Immunogen Species
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Has a preference for oxidized pyrimidines, such as thymine glycol (prefers 5S isomers) 5,6-dihydrouracil:G, 5-hydroxyuracil:G, 5-hydroxycytosine:G and urea:A. Cleaves ssDNA containing an AP site.
Protein Families
Nth/MutY family
Database Links

KEGG: mtu:Rv3674c

STRING: 83332.Rv3674c

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