ENT1 Antibody

^{Rare Species}

Datasheet

Code	CSB-PA142286XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: ENT1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q8VXY7

Target Names

ENT1

Alternative Names

Equilibrative nucleotide transporter 1 (AtENT1) (Nucleoside transporter ENT1) ENT1 At1g70330 F17O7.13

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ENT1 protein (255-299aa)

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The generation of the ENT1 polyclonal antibody begins with the selection of the recombinant Arabidopsis thaliana ENT1 protein (255-299aa) as the immunogen. This protein is used to immunize a rabbit, inducing an antibody response. Subsequently, serum is collected from the rabbit to obtain polyclonal antibodies, which are then purified through affinity chromatography. The ENT1 antibody can react with Arabidopsis thaliana ENT1 protein, and its functionality has been validated through ELISA and WB assays.

The main function of Arabidopsis thaliana ENT1 protein is to transport nucleosides across the cell membrane. It plays a crucial role in the uptake and distribution of nucleosides, which are essential for various cellular processes such as DNA and RNA synthesis, energy metabolism, and signaling pathways. ENT1 protein helps maintain the balance of nucleosides inside the cell and facilitates their utilization in different cellular functions.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Nucleoside transporter involved in adenosine transport and required for nucleotide metabolism which influences growth and pollen germination. Has high affinity for adenosine when expressed in a heterologous system (yeast).

Gene References into Functions

ENT1-mediated nucleosides, especially adenosine transport, is important for nucleotide metabolism, thus influencing growth and pollen germination. PMID: 21642237

Subcellular Location

Vacuole membrane; Multi-pass membrane protein. Note=Tonoplast.

Protein Families

SLC29A/ENT transporter (TC 2.A.57) family

Tissue Specificity

In young seedlings, expressed in root elongation zone, root cortex, root-hair, at the transition to the shoot and cotyledons. Expressed in hydathodes of fully developed leaves and pollen.

Database Links

KEGG: ath:AT1G70330

STRING: 3702.AT1G70330.1

UniGene: At.21128

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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