entD Antibody

Datasheet

Code	CSB-PA262984XA01ENV
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) entD Polyclonal antibody

Uniprot No.

P19925

Target Names

entD

Alternative Names

entD antibody; b0583 antibody; JW5085Enterobactin synthase component D antibody; 4'-phosphopantetheinyl transferase EntD antibody; EC 2.7.8.- antibody; Enterochelin synthase D antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) entD protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron(2+) atoms. Plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively.

Gene References into Functions

Data suggest that EntD, a phosphopantetheinyl transferase, participates in the biosynthesis of the siderophore yersiniabactin. PMID: 23853582
Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone. PMID: 20636978

Subcellular Location

Membrane.

Protein Families

P-Pant transferase superfamily, EntD family

Database Links

KEGG: ecj:JW5085

STRING: 316385.ECDH10B_0650

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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