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entF Antibody

Datasheet
Code CSB-PA320000XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) entF Polyclonal antibody
Uniprot No.
P11454
Target Names
entF
Alternative Names
entF antibody; b0586 antibody; JW0578Enterobactin synthase component F antibody; EC 2.7.7.- antibody; Enterochelin synthase F antibody; Serine-activating enzyme antibody; Seryl-AMP ligase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
A synthesized peptide derived from Escherichia coli (strain K12) entF protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, Dot blot (guaranteed specific to the immunizing peptide)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;
Quality Guarantee
1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

entF Proteins

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Target Background

Function
Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE).
Gene References into Functions
  1. mutants in entF did not produce active microcin E492 in the presence of enterochelin or salmochelin. PMID: 18502859
Protein Families
ATP-dependent AMP-binding enzyme family, EntF subfamily
Database Links
Promotions
Reward For Reviews
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