EPF1 Antibody

Datasheet

Code	CSB-PA848284XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EPF1 Polyclonal antibody

Uniprot No.

Q8S8I4

Target Names

EPF1

Alternative Names

EPF1 antibody; At2g20875 antibody; F5H14Protein EPIDERMAL PATTERNING FACTOR 1 [Cleaved into: MEPF1] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EPF1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Controls stomatal patterning. Regulates asymmetric cell division during guard cell differentiation. Mediates stomatal development inhibition. Not cleaved by the protease CRSP (AC Q9LNU1). MEPF1: mobile signal controlling stomatal development in a non-cell-autonomous manner. Uses ERL1 as major receptor. May act by competing with somatogen (AC Q9SV72) for the same receptor, TMM (AC Q9SSD1).

Gene References into Functions

Here, the authors report that EPF1 and its primary receptor ERECTA-LIKE1 (ERL1) target MUTE, a transcription factor specifying the proliferation-to-differentiation switch within the stomatal cell lineages. In turn, MUTE directly induces ERL1. PMID: 28266915
EPF1/EPF2-like proteins form an additional disulfide bond in their loop regions and show greater flexibility in these regions than EPFL9/Stomagen-like peptides. PMID: 23755192
EPF1 and EPF2 are able to activate the MAP kinase MPK6, and that both EPF1 and EPF2 are able to decrease the SPCH level, whereas stomagen is able to increase it. PMID: 23686240

Subcellular Location

Secreted.

Protein Families

Plant cysteine rich small secretory peptide family, Epidermal patterning factor subfamily

Tissue Specificity

Expressed in shoots, but not in roots. Mostly localized in developing leaves, specifically in meristemoids, guard mother cells (GMCs), and young guard cells.

Database Links

KEGG: ath:AT2G20875

STRING: 3702.AT2G20875.1

UniGene: At.52849

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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EPF1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background